LOCUS       40924_5154       13862 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAY205 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Scholz P, Haring V, Wittmann-Liebold B, Ashman K, Bagdasarian M, 
            Scherzinger E.
  TITLE     Complete nucleotide sequence and gene organization of the 
            broad-host-range plasmid RSF1010
  JOURNAL   Gene 75 (2), 271-288 (1989)
  PUBMED    2653965
REFERENCE   2  (bases 1 to 13862)
  AUTHORS   Nishikawa M, Suzuki K, Yoshida K.
  TITLE     Structural and functional stability of IncP plasmids during stepwise
            transmission by trans-kingdom mating: promiscuous conjugation of 
            Escherichia coli and Saccharomyces cerevisiae
  JOURNAL   Jpn. J. Genet. 65 (5), 323-334 (1990)
  PUBMED    2248784
REFERENCE   3  (bases 1 to 13862)
  AUTHORS   Nishikawa M, Suzuki K, Yoshida K.
  TITLE     DNA integration into recipient yeast chromosomes by trans-kingdom 
            conjugation between Escherichia coli and Saccharomyces cerevisiae
  JOURNAL   Curr. Genet. 21 (2), 101-108 (1992)
  PUBMED    1568253
REFERENCE   4  (bases 1 to 13862)
  AUTHORS   Moriguchi K, Edahiro N, Yamamoto S, Tanaka K, Kurata N, Suzuki K.
  TITLE     Transkingdom genetic transfer from Escherichia coli to Saccharomyces
            cerevisiae as a simple gene introduction tool
  JOURNAL   Appl. Environ. Microbiol. 79 (14), 4393-4400 (2013)
  PUBMED    23666333
REFERENCE   5  (bases 1 to 13862)
  AUTHORS   Nishikawa M, Moriguchi K, Suzuki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2009) Contact:Katsunori Suzuki Hiroshima 
            University, Department of Biological Science, Graduate School of 
            Science; Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 739-8526, 
            Japan
REFERENCE   6  (bases 1 to 13862)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 13862)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1989"; volume: "75"; issue: "2"; pages: "271-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Jpn. J. 
            Genet."; date: "1990"; volume: "65"; issue: "5"; pages: "323-334"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Curr. 
            Genet."; date: "1992"; volume: "21"; issue: "2"; pages: "101-108"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "14"; 
            pages: "4393-4400"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2009) Contact:Katsunori Suzuki Hiroshima University, 
            Department of Biological Science, Graduate School of Science; 
            Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 739-8526, Japan"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          9762..9869
                     /mol_type="other DNA"
                     /db_xref="taxon:2504"
                     /organism="Plasmid RSF1010"
     rep_origin      complement(396..790)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(817..1101)
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC"
                     /label=mobC
                     /protein_id="BAI47939.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(817..1101)
                     /gene="mobC"
                     /label=mobC
     oriT            1132..1219
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2048..2461
                     /codon_start=1
                     /gene="mobB"
                     /product="MobB"
                     /label=mobB
                     /protein_id="BAI47941.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            2048..2461
                     /gene="mobB"
                     /label=mobB
     CDS             2458..3426
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             3490..3702
                     /codon_start=1
                     /product="unknown protein E"
                     /label=unknown protein E
                     /note="unknown protein E gene"
                     /protein_id="BAI47943.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             3704..3910
                     /codon_start=1
                     /product="repressor protein F"
                     /label=repressor protein F
                     /note="repressor protein F gene"
                     /protein_id="BAI47944.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             3940..4776
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             4766..5614
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     misc_feature    5821..6137
                     /gene="bla"
                     /label=beta-lactamase, C-terminus region
                     /note="beta-lactamase, C-terminus region"
     gene            5821..6137
                     /gene="bla"
                     /label=bla
     rep_origin      6302..6847
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             7442..8254
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8544..9035
                     /gene="tnpR"
                     /label=transposon Tn3 resolvase, C-terminus region
                     /note="transposon Tn3 resolvase, C-terminus region"
     gene            8544..9035
                     /gene="tnpR"
                     /label=tnpR
     promoter        9113..9217
                     /label=AmpR promoter
     misc_feature    9218..9761
                     /gene="bla"
                     /label=beta-lactamase, N-terminus region
                     /note="beta-lactamase, N-terminus region"
     gene            9218..9761
                     /gene="bla"
                     /label=bla
     rep_origin      complement(9873..10710)
                     /direction=LEFT
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     promoter        complement(11224..11325)
                     /label=TRP1 promoter
     promoter        11333..11361
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             11409..12596
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        12760..12980
                     /label=URA3 promoter
     CDS             12981..13781
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      13785..13862
                     /gene="URA3"
                     /label=URA3 terminator
                     /note="URA3 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc
       61 tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc
      121 tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag
      181 agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc
      241 gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc
      301 tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg
      361 ccagccagtg cccgtcactc ccgtactaac tgtcacgaac ccctgcaata actgtcacgc
      421 ccccctgcaa taactgtcac gaacccctgc aataactgtc acgcccccaa acctgcaaac
      481 ccagcagggg cgggggctgg cggggtgttg gaaaaatcca tccatgatta tctaagaata
      541 atccactagg cgcggttatc agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc
      601 cggccagagg ccggatagct ggtctattcg ctgcgctagg ctacacaccg ccccaccgct
      661 gcgcggcagg gggaaaggcg ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat
      721 acgctggagc gcttttagcc gctttagcgg cctttccccc tacccgaagg gtgggggcgc
      781 gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag cccggctcat ccttctggcg
      841 tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc
      901 catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac
      961 cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg
     1021 ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc
     1081 cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat
     1141 catggaggca cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg
     1201 tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca
     1261 ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg
     1321 gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag
     1381 cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg
     1441 ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc
     1501 aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag
     1561 cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat
     1621 acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag
     1681 cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag
     1741 accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag
     1801 cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac
     1861 gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac
     1921 ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg
     1981 gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca
     2041 atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga
     2101 gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat
     2161 gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca
     2221 gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag
     2281 cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac
     2341 cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct
     2401 cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg
     2461 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
     2521 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
     2581 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
     2641 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
     2701 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
     2761 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     2821 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     2881 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     2941 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     3001 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     3061 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     3121 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     3181 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     3241 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     3301 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     3361 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     3421 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     3481 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
     3541 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
     3601 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
     3661 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
     3721 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
     3781 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
     3841 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
     3901 cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc
     3961 aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg
     4021 gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc
     4081 ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc
     4141 accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg
     4201 cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg
     4261 atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc
     4321 aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc
     4381 gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc
     4441 gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg
     4501 ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc
     4561 tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac
     4621 gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc
     4681 gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg
     4741 cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca
     4801 cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg
     4861 cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga
     4921 gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc
     4981 catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca
     5041 gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgaatgcc atgtggtcaa
     5101 aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct
     5161 caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg
     5221 ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg
     5281 cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt
     5341 gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca
     5401 gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg
     5461 cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt
     5521 gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg
     5581 caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca
     5641 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     5701 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     5761 gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct tctgctcctg
     5821 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttaata agatgatctt
     6301 cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc
     6361 agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
     6421 gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca
     6481 agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt
     6541 tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg
     6601 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     6661 ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     6721 aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6781 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     6841 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct
     6901 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag
     6961 cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc
     7021 accggtgcag ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc
     7081 caacatagta agccagtata cactccgcta gcgctgaggt ctgcctcgtg aagaaggtgt
     7141 tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg
     7201 gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac
     7261 ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg
     7321 atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa
     7381 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     7441 tatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga
     7501 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     7561 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     7621 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     7681 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     7741 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     7801 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     7861 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     7921 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     7981 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     8041 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     8101 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     8161 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     8221 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     8281 acactggcag agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact
     8341 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     8401 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     8461 tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct
     8521 tcttcacgag gcagacctca gcgctcaaag atgcaggggt aaaagctaac cgcatcttta
     8581 ccgacaaggc atccggcagt tcaacagatc gggaagggct ggatttgctg aggatgaagg
     8641 tggaggaagg tgatgtcatt ctggtgaaga agctcgaccg tcttggccgc gacaccgccg
     8701 acatgatcca actgataaaa gagtttgatg ctcagggtgt agcggttcgg tttattgacg
     8761 acgggatcag taccgacggt gatatggggc aaatggtggt caccatcctg tcggctgtgg
     8821 cacaggctga acgccggagg atcctagagc gcacgaatga gggccgacag gaagcaaagc
     8881 tgaaaggaat caaatttggc cgcaggcgta ccgtggacag gaacgtcgtg ctgacgcttc
     8941 atcagaaggg cactggtgca acggaaattg ctcatcagct cagtattgcc cgctccacgg
     9001 tttataaaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     9061 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9421 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     9481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagagcttg ccgccgccgc
     9781 aggtggagct gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt
     9841 attggcggcg ctgaaagaaa ccgcaagaat tcccacatgt taaaatagtg aaggagcatg
     9901 ttcggcacac agtggaccga acgtggggta agtgcactag ggtccggtta aacggatctc
     9961 gcattgatga ggcaacgcta attatcaaca tatagattgt tatctatctg catgaacacg
    10021 aaatctttac ttgacgactt gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa
    10081 tatgtgtcac tgtttgatat tactgtcagc gtagaagata atagtaaaag cggttaataa
    10141 gtgtatttga gataagtgtg ataaagtttt tacagcgaaa agacgataaa tacaagaaaa
    10201 tgattacgag gatacggaga gaggtatgta catgtgtatt tatatactaa gctgccggcg
    10261 gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg tatgcgcctg
    10321 tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag tcaaccccct
    10381 gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca ttatctttac
    10441 atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc aaaaggcctg
    10501 caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct atttcttagc
    10561 atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc
    10621 gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt
    10681 cagtccacca gctaacataa aatgtaagct ttcggggctc tcttgccttc caacccagtc
    10741 agaaatcgag ttccaatcca aaagttcacc tgtcccacct gcttctgaat caaacaaggg
    10801 aataaacgaa tgaggtttct gtgaagctgc actgagtagt atgttgcagt cttttggaaa
    10861 tacgagtctt ttaataactg gcaaaccgag gaactcttgg tattcttgcc acgactcatc
    10921 tccatgcagt tggacgatat caatgccgta atcattgacc agagccaaaa catcctcctt
    10981 aggttgatta cgaaacacgc caaccaagta tttcggagtg cctgaactat ttttatatgc
    11041 ttttacaaga cttgaaattt tccttgcaat aaccgggtca attgttctct ttctattggg
    11101 cacacatata atacccagca agtcagcatc ggaatctaga gcacattctg cggcctctgt
    11161 gctctgcaag ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga
    11221 catactccaa gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca
    11281 atgccctccc tcttggccct ctccttttct tttttcgacc gaattctcat gtttgacagc
    11341 ttatcatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
    11401 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
    11461 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt
    11521 ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc
    11581 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
    11641 cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga
    11701 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    11761 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    11821 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    11881 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    11941 gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca
    12001 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
    12061 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
    12121 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
    12181 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
    12241 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
    12301 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
    12361 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
    12421 gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga
    12481 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
    12541 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
    12601 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
    12661 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
    12721 catctccagc agccgcacgc ggcgcatccc cccccccctt tcaattcaat tcatcatttt
    12781 ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg gtaatctccg
    12841 aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta
    12901 gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct
    12961 gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc
    13021 ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg
    13081 cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca
    13141 aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag
    13201 ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat
    13261 ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag
    13321 aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga
    13381 agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt
    13441 catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga
    13501 gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag
    13561 gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg
    13621 gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg
    13681 gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag
    13741 caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag
    13801 taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
    13861 cc
//
